CellMaker Mammalian System

The CellMaker MAMMALIAN SYSTEM is a state-of-the-art, easy to use, single use bioreactor system which includes a wealth of new technologies and allows growth of the widest range of cell types. It is a fully automated system designed specifically for mammalian and insect cell culture but can also be used for pichia, E. coli, algae and bacteriophage.

Whilst other disposable systems require shakers, rockers, rollers or large environmental chambers, the CellMaker MAMMALIAN SYSTEM employs novel airlift technology, available only from Cellexus Limited and already proven in the CellMaker Regular for pichia, E.coli, algae and bacteriophage culture. The methodology is simple, effective, scalable and inexpensive to use. Coupling of this unique airlift technology with non-invasive, impellor stirrers allow the cell culture to be very gently mixed to deliver very efficient and controllable aeration, with minimal sheering whilst eliminating cell settling.

The CellMaker MAMMALIAN SYSTEM comprises of the CellMaker MAMMALIAN SYSTEM Controller and the CellMaker MAMMALIAN SYSTEM Enclosure. Cells are grown with fast set-up times and no cleaning requirements. When the CellexusBag is placed in the CellMaker MAMMALIAN SYSTEM it is very efficiently aerated, with controlled air flow providing oxygen for cell growth. The non-invasive mixing also cells are maintained in suspension. Pressure can be controlled to enhance oxygen availability to cells and decrease foaming. Temperature control also enables production of the optimal yields of soluble protein under the most favourable conditions. The CellMaker MAMMALIAN SYSTEM can be used for culture of almost any cell type, including mammalian and insect cells, with full control of dissolved oxygen and carbon dioxide concentration with pH monitoring and control.

Control of dO2 is essential to achieve optimal cell concentrations and viabilities for mammalian and insect cells so the system incorporates integrated oxygen sensors which are used to measure dissolved oxygen concentration. Data is fed-back to the CellMaker MAMMALIAN SYSTEM Controller, which regulates and adjusts flow of air, nitrogen and carbon dioxide to the cells. Too little oxygen and cells may not achieve concentrations required for seeding large-scale bioreactors whilst too much oxygen and the cells may undergo apoptosis.

The culture of pichia, E. coli, algae and baceriophage is very straightforward in the CellMaker MAMMALIAN SYSTEM and can be performed without need for automated dO2 or pH control. The CellMaker MAMMALIAN SYSTEM with the CellexusBag can achieve oxygen mass transfer rates similar to a stirred bioreactor and considerably greater than a shaker flask to provide optimal protein yields.

CellMaker MAMMALIAN SYSTEM - Main Features and Benefits

Flexibility and Performance:

Easy to buy (various options available), install and use – provides simplicity, less labour intensive and enables fast set up times
Disposable, single-use bioreactor specifically engineered for mammalian and insect cell culture but also suitable for other cell types - provides optimal protein production across all cell types, even those which are more challenging
Precise control of culture temperature – eliminates need for external environment regulation
Couples airlift technology and gentle impellor stirring - delivers very efficient and controllable aeration and mixing, with minimal sheering and eliminating cell settling
Single-use, disposable, non-penetrative sensor technology - efficiently monitors and controls dO2 and pH to give optimal growth conditions, cell type by cell type, and production of optimal ODs of soluble protein
Simple set up procedure and systems programming - allows cultures to be set up rapidly (<60mins)
Single-use culture bags - eliminates cleaning requirements and minimizes down-time
Automated delivery of acid/base and/or control of external CO2 supply – provides stringent pH control
Higher performance than shaker flasks and comparable performance to steel/glass stirred systems – optimal yields with ease of use
Connects to bioreactor cell culture bags via autoclavable, reusable sterile filters - minimises running costs
Flexible culture/sample ports - easy inoculation and removal of culture samples
Single-use CellexusBag manufactured under ISO 10,000 conditions and system built to ISO 9,000 (complies with 21Cfr part 21) - eliminates cleaning requirements, minimises down-time and provides quality assurance
Data logging capabilities compatible with many commonly used systems such as DeltaV – enables simple incorporation into existing processes

Use:

Applies single-use, disposable bioreactor bags – provides flexibility for use with all cell types, eliminates cross-contamination and minimises system down-time
No cleaning needed - no time spent validating cleaning and reduces down-time
No autoclaving – enables use straight from the box and reduces down-time
Inexpensive to use – reduces costs
Easy to operate – no computer software to install or programs to learn and minimal installation time to reduce training time/costs
Easy to buy – ask about our rapid access rental plans

Downloads of data sheets are also available

For more information download a product summary sheet (LINK TO PDF OF PRODUCT FLYER)

Ordering Information >

Back to the page top ^