CellMaker PLUS

The CellMaker PLUS is:

A state-of-the-art, easy to use, single-use, disposable bioreactor cell culture control instrument.
Fully automated and includes a wealth of new technologies for efficient growth of the widest range of cell types including E. coli, pichia, algae and bacteriophage as well as the more challenging mammalian and insect cells.
Does not require mechanical methods to mix or oxygenate. Whilst other disposable systems require shakers, rockers, rollers or large environmental chambers, the CellMaker PLUS employs a novel airlift technology, available only from Cellexus, which is simple, scalable and inexpensive to use.

The CellMaker PLUS includes four components:

SupplyHub incorporating the air pumping system, temperature regulation and data aquisition.
Controller to enable important user defined parameters to be specified. The unit is modular and can be provided with / without DO and pH sensor options. This enables use with the broadest range of cell types, including mammalian and insect cells as well as E.coli, pichia and algae.
Enclosure in which the CellexusBag is held and which incorporates a Peltier heater to enable cell culture temperature to be closely monitored and controlled. Both 8L and 50L Enclosures are available to provide flexibility in scale.
Oxygenator which can dovetail with the SupplyHub to provide continuous supply of oxygen and which ia also available as a stand-alone unit.

It uses the unique CellexusBag in which cells are grown, with fast set-up times, no cross contamination and no cleaning requirements. When the CellexusBag is placed in the CellMaker PLUS Enclosure it is very efficiently aerated, with controlled air flow providing oxygen for cell growth and gentle, non-invasive effective mixing to maintain cells in suspension. Pressure can be controlled via the CellMaker PLUS Controller to enhance oxygen availability to cells and decrease foaming. Temperature control also enables production of the highest yields of soluble protein under optimal conditions.
Control of DO is essential to achieve optimal cell concentrations and viabilities for mammalian and insect cells so the CellMaker PLUS Controller is available with optional integrated pH and oxygen sensors which are used to measure pH levels and dissolved oxygen concentration. Data is fed-back to the CellMaker PLUS Controller, which controls pH and regulates and adjusts flow of air, oxygen and carbon dioxide to the cells. Too little oxygen and cells may not achieve concentrations required for seeding large-scale bioreactors whilst too much oxygen and the cells may undergo apoptosis.

CellMaker PLUS System - Main Features and Benefits

Flexibility and Performance:

Easy to buy (various options available), install and use – provides simplicity, less labour intensive and enables fast set up times
Simple set up procedure and systems programming - allows cultures to be set up rapidly (<30mins)
Single-use culture bags - eliminates cleaning requirements, cross contamination and minimises down-time
Provides highly accurate control conditions including temperature control (15°C - 40°C) with built-in chiller – no external chilling/heating equipment needed and gives optimal production of higher ODs of soluble protein
Oxygen generation capability (up to 4 litres / minute) - eliminates need, and extra cost, of gas
Higher efficiency of oxygen transfer - potential of protein production at higher ODs
Efficient airlift technology - delivers very efficient, controllable aeration and mixing, with minimal sheering and eliminating cell settling
Higher performance than shaker flasks and comparable performance to steel/glass stirred systems – optimal protein yields with ease of use
Connects to bioreactor cell culture bags via autoclavable, reusable sterile filters - minimises running costs
Programmable air/enriched air flow rate (1-10 litres per minute) – provides very efficient mixing and aeration to improve protein yields
Flexible culture/sample ports - easy inoculation and removal of culture samples
Single-use CellexusBag manufactured under ISO 10,000 conditions and system built to comply with ISO 9,000 - eliminates cleaning requirements, minimises down-time and provides quality assurance

Optional

Single-use, disposable, non-penetrative sensor technology - efficiently monitors and controls pO2 and pH to give optimal growth conditions, cell type by cell type, and production of optimal yields of soluble protein
Automated delivery of external CO2 supply – provides stringent pH control

Use:

Easy to buy, install and use – less labour intensive and reduces set up times (< 30 minutes)
Inexpensive to use – reduces costs
Available in two sizes: 8 litre and 50 litre - flexibility in scale
Applies single-use, disposable CellexusBag bioreactor bags – provides flexibility for use with all cell types, eliminates cross-contamination and minimises system down-time
No cleaning needed - no time spent validating cleaning and reduces down-time
No autoclaving – enables use straight from the box and reduces down-time
Inexpensive to use – reduces costs
User friendly, adaptable software – quick to set up but provides flexibility
Easy to buy – ask about our rapid access rental plans

Downloads of data sheets are also available

For more information download a product summary sheet

Ordering Information >

CellMaker PLUS Dimensions / Specification

SupplyHub:
Dimensions: H 506.0mm (19.92inches) W 300.0mm (11.81 inches) D 572.0mm (22.52 inches)
Dual Input Voltage: AC 110 V or AC 230V
Mains Rated Power Supply: 16A @ 110V / 13A @ 230V
Mains Input Lead: IEC C19 16A 2 meter mains lead
Linear oil-free pump delivering 0-20lpm air
Incorporates USB Data Acquisition Module

Controller:
Dimensions: H 506.0mm (19.92inches) W 300.0mm (11.81 inches) D 572.0mm (22.52 inches)
Input Voltage: via SupplyHub connection
Mains Rated Power Supply: N/A (power supplied from SupplyHub)
Mains Input Lead: N/A (power supplied from SupplyHub)
User interface via 15” Touchscreen monitor with 1024x768 resolution
Incorporates mini-PC loaded with Bespoke Labview software
PC Connectivity: 6 x USB ports, Ethernet LAN, Mini SD socket
Reader device (optional) for disposable fluorescent DO and pH probes (available integrated within CellexusBags).
DO sensitivity: 0-500% Air (temperature compensated); accuracy <0.3% Air sat
pH range: 5.5-10; accuracy 0.02 pH units

Enclosures:
8 litre Enclosure Dimensions: H 541.0mm (21.30inches) W 300.0mm (11.81inches) D 353.0 mm (13.90inches)
Input Voltage: via SupplyHub connection
Mains Rated Power Supply: N/A (power supplied from SupplyHub)

Mains Input Lead:
N/A (power supplied from SupplyHub)
Gas Mixing: Air / Oxygen – (supplied from SupplyHub)

Oxygenator:
Dimensions: H 506.0mm (19.92inches) W 300.0mm (11.81 inches) D 572.0mm (22.52 inches)
Dual Input Voltage: AC 110 V or AC 230V
Pressure Swing Absorption (PSA) based process
Delivering a continuous supply of oxygen at up to 4lpm
Mains Rated Power Supply: 16A @ 110V / 13A @ 230V
Mains Input Lead: IEC C19 16A 2 meter mains lead

Overall system quality:
Fully CE-marked, tested to laboratory safety standard BS EN61010, and EMC standard (BS EN61326) to satisfy Low Voltage Directive (2006/95/EC) and Elecro Magnetic Compatibility Directive (2004/108/EC).

Downloads of data sheets are also available

For more information download a product summary sheet

Ordering Information >